Liliana Viasu
Teaching Hospital of Nephrology dr.Carol Davila
Romania
Title: Chicken immunological active proteins (CIAP). The specific reaction against multiple resistance bacterial strains in urinary tract infections
Biography
Biography: Liliana Viasu
Abstract
Statement of the Problem: The emergence of multidrug resistant bacteria in urinary tract infections (UTIs) is a challenge to medical professionals. According to ECDC (2018), more than a third of K. pneumoniae and half of E. coli strains reported were resistant to at least one of the antibiotics under surveillance. Moreover, the emergence of Colistin resistance among Enterobacteriaceae leave few therapeutic options against these”superbugs”. Thus, immunological active proteins (IAP) have emerged as a potential therapeutic agent.
Material and methods: In this study, CIAP (egg proteins: IgY, holo-ovotransferrin, ovomucin, ovoalbumin and lysozyme) were obtained from Rhode Island red chickens immunized with antigens from E. coli, Klebsiella pneumoniae, Enterococcus spp, Pseudomonas sp, Proteus sp, Candida sp, and MRSA strains. 90 patients were included in the study, 87 of them compliant with the treatment; 20 were inpatients and 67 outpatients at Teaching Hospital of Nephrology dr.Carol Davila, Bucharest. 65% of them were known to have recurrent UTIs. The etiology of the UTIs: 68% E. coli, 12.6% multiple bacteria, 11.5% Klebsiella, 7.9% others. 35% of the isolates were MDR. CIAP efficiency was demonstrated in vitro by: quantitative assay for Chicken IgY ELISA Kit - ABCAM, rapid and slow agglutination test and bacterial growth inhibition test - HB&L ALIFAX (IAP + live bacterial cultures). CIAP were administered at diagnosis together with antibiotics, and as follow-up treatment for an average of 2 months.
Results: 8 patients were non-responsive (7F+1M), 79 were responsive as follows: 90% of the patients were cured with no recurrence, 10% had one recurrence during the study. For these patients, personalized treatment was made, using the strain isolated by urine culture after recurrence; the new treatment was curative.
Conclusion: Preliminary results reveal the possibility of using passive immunity to stimulate the active immunity in preventing infection recurrence and antibiotic resistance.